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Add 250 mL of dH2O to a graduated cyclindar. 2. Weigh out 20g of premix LB Agar powder (VWR DF0445-17) or: 3. 5.0 g tryptone 4. 2.5 g yeast extract 5. 5.0 g NaCl 6. 7.5 g agar 7.

Agar plate recipe

Autoclave 15 g agar in 800 ml water for 15 min. Add sterile concentrated minimal medium and carbon source. After Refer to CLEAPSS Recipe card 1 for full details of handling agar. Handbook section 15.2.7 has more information on how to manage agar and prepare plates. Pour 10 mL of agar into each petri dish. The 60 mL syringe will only allow the pouring of 6 dishes.

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5 g Jan 3, 2021 But there are many different recipes to prepare growth media for bacteria, as some bacterial species require different combinations of nutrients. Aug 27, 2013 Summary: Bacteria need nutrient plates to grow on. This protocol will show you how to make selective.

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Measure agar and distilled water into clean flask or beaker. · Flame sterilize a clean glass stir rod to stir the medium as it melts. · While wearing heat resistant hand TxF “Special Recipe” with Charcoal – Pre-Poured Agar Plates (MEA). $25.00 – $ 150.00. Unit Size.
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1. Add 250 mL of dH2O to a graduated cyclindar. 2. Weigh out 20g of premix LB Make your own agar Petri dishes and grow bacterial colonies.

Incubate inverted plate at organism's ideal temperature for 24 to 48 hours. Batch makes about 40 plates.
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How to make LB agar plates Weigh out 7.5 g agar, 5 g tryptone, 5 g sodium chloride (NaCl) and 2.5 g yeast extract and add to a 1 L Duran bottle. Measure out approximately 400 mL of distilled water and add to the Duran bottle.

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For professional use only. Do not use plates if they show evidence of microbial contamination, discoloration, drying, cracking or other signs of deterioration. Consult GENERAL INSTRUCTIONS FOR USE document for aseptic handling procedures, MacConkey Agar is a modified neutral red bile salt agar by Alfred Theodore MacConkey. It is one of the earliest methods used to cultivate and identify enteric organisms.